Description:

Roti®-PVDF 2.0, pore size 0,2 µm roll, 300 x 30 cm, PVDF-membr. PVDF membrane with 0,2 µm pore size Binding capacity for proteins: ≥125 µg/cm2 Roti®-PVDF 0.2 is a hydrophobic, microporous polyvinylidene membrane, free from adjuvants and detergents. It has been optimised for transfer and highly sensitive detection of proteins of up to 70 kDa, but is also perfectly suited for blotting of bigger proteins. The membrane shows superior protein binding parameters and stands out for its particularly low background. It may also be used very well after repeated stripping and harsh washing processes. This mechanically rigid transfer membrane can be easily cut to the desired gel size and it remains flexible and tear-proof even when dry. Briefly immerse Roti®-PVDF 0.2 membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every blotting system. Membrane thickness: 140 ± 20 µm Pore size: 0,2 µm Due to production processes, the membrane shows two different sides – one smooth and one raw. For optimally distinct bands, please use the smooth side for protein binding. Applications: optimised for proteins of ≤70 kDa. Western-blot, Dot-blot, lipopolysaccharide- and amino acid analysis, bindings assays, N-terminal peptide sequencing. Detection systems: chemoluminescence, radioactivity, colour

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Weight	0.851 kg