Description:

Prepacked ROTI®Dex-Grav columns for size exclusion chromatography by gravity, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.

The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.

In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.
In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.
Advantages:

Sample preparation in just a few minutes
Desalting, removal of impurities and buffer exchange in a single step
No interaction between analyte and medium
High selectivity, high resolution and high yield (> 95%)
Also suitable for small sample volumes
High chemical stability and therefore compatible with many organic and other solvents

Size Exclusion Chromatography Columns ROTI®Dex-25 Medium Grav XS

The column bed consists of ROTI®Dex-25 Medium (Art. No. 21A5), pre-swollen in deionised water.
Suitable for:

Oligonucleotides > 10 bp
Globular proteins > 5 kDa
Particles > 2 nm

Column size XS is recommended for sample volumes of 0.15-0.3 ml.

Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.

Technical Information
Version Grav XS
Matrix Cross-linked dextran
MWCO 5 kDa
10 bp
Particle size Dry: 50-150 µm
Wet: 85-260 µm
Storage buffer Deionized water with 0.15% ProClin® 150
Sample volume 0.15-0.3 ml
Elution volume 0.35 ml

Lead Times

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