Description:

Happy Cell ASM for Flow Cytometry. Formulated in PBS without magnesium and calcium, 20ml. This reagent offers an excellent solution to passive cell sedimentation in flow cytometry.

Cell sedimentation is a common problem faced by cell cytometrists. This can occur when cells are large or are left to stand for long periods of time. Under these conditions, cells can frequently sediment out of solution, which can lead to inconsistent cell disperision. This can negatively impact experimental results. For example, larger cells in a population will sediment at a faster rate than smaller cells. This in turn will lead to a higher percentage of smaller cells being sampled. As a result, unrepresentative sub-population data may be obtained causing inaccurate or inconsistent experimental results and conclusions. To support research in the field of flow cytometry, Vale Life Sciences have developed a suspension system specifically for this application.

The media base is Phosphate buffered saline (PBS) without divalent cations, magnesium, or calcium. If required, these or any other supplement can be added. HappyCell® ASM Flow Cytometry reagent has been formulated to be compatible with commercially available flow cytometers. This product is supplied as a 20ml 2X concentrate, and is readily diluted with standard PBS. Simply re-suspend cells at desired density and proceed with the flow cytometry protocol.